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目的:探讨草苁蓉环烯醚萜苷(IGBR)对兔VX2移植瘤的生长抑制作用和血清抗氧化活性的影响。方法:建立兔皮下VX2移植瘤模型,将实验动物分为模型组、IGBR高、低剂量组和氟尿嘧啶(5-Fu)组,观察各组荷瘤兔的生存情况。另选一批实验动物,连续ig给药21 d后处死动物,剥离肿瘤组织,称瘤重和计算抑瘤率,并比色法检测血清谷胱甘肽-S-转移酶(GST)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活性及丙二醛(MDA)含量。结果:与模型组相比,IGBR能显著延长VX2荷瘤兔生存时间,提高其生命延长度;显著减小移植瘤瘤重,提高抑瘤率。同时,IGBR可明显升高血清SOD,CAT和GSH-PX活性,降低MDA水平。结论:IGBR可提高VX2荷瘤兔生存时间,对VX2移植瘤具有明显的抑制作用,其作用可能与其增高机体抗氧化能力有关。
OBJECTIVE: To investigate the inhibitory effect of iridoid glycosides (IGBR) on rabbit VX2 xenograft tumor growth and serum antioxidant activity. METHODS: A subcutaneous VX2 xenograft tumor model was established in rabbits. Experimental animals were divided into model group, IGBR high and low dose group and fluorouracil (5-Fu) group. The survival of rabbits in each group was observed. An additional batch of experimental animals was selected and sacrificed 21 days after continuous ig administration. The animals were sacrificed and the tumor tissue was detached. The tumor weight was calculated and the tumor inhibition rate was calculated, and serum glutathione-S-transferase (GST) and ultrasonography were measured by colorimetry. Oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX) activity and malondialdehyde (MDA) content. RESULTS: Compared with the model group, IGBR significantly prolonged the survival time of VX2 tumor-bearing rabbits and increased the length of life of the rabbits; it significantly reduced the tumor weight of transplanted tumors and increased the tumor inhibition rate. At the same time, IGBR significantly increased serum SOD, CAT and GSH-PX activity, and decreased MDA levels. Conclusion: IGBR can increase the survival time of VX2 tumor-bearing rabbits, and has a significant inhibitory effect on VX2 tumors. The effect of IGBR may be related to its increased antioxidant capacity.