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目的探索放线菌酮和ＴＮＦα协同诱导人大肠癌Ｌｏｖｏ细胞凋亡的发生规律．方法用细胞体外培养方法，以放线菌酮和ＴＮＦα均小剂量作用于人大肠癌Ｌｏｖｏ细胞，经Ｈｏｅｃｈｓｔ３３２５８荧光染色，光镜观察形态改变，并用流式细胞术检测ＤＮＡ断裂情况．结果单用ＴＮＦα５×１０５Ｕ／Ｌ或放线菌酮４ｍｇ／Ｌ，均未引起Ｌｏｖｏ细胞凋亡效应．而两者协同，随作用１２ｈ～４８ｈ，Ｌｏｖｏ细胞渐出现明显的凋亡形态学改变和ＤＮＡ断裂．结论小剂量的放线菌酮和ＴＮＦα协同作用于人大肠癌Ｌｏｖｏ细胞，出现明显的凋亡效应．
Objective To explore the synergistic effect of cycloheximide and TNFα on the apoptosis of human colon cancer Lovo cells. Methods Human colon cancer cells Lovo cells were treated with cycloheximide and TNFα at low doses by cell culture in vitro. The cells were stained with Hoechst 33258, the morphology was observed by light microscopy, and DNA fragmentation was detected by flow cytometry. Results The single use of TNFα5×105U/L or cycloheximide 4mg/L did not induce Lovo cell apoptosis. The synergistic effect of the two, with the role of 12h ~ 48h, Lovo cells gradually appeared apoptotic morphological changes and DNA breaks. Conclusion Low doses of cycloheximide and TNFα act synergistically on human colon cancer Lovo cells and show obvious apoptosis effect.