论文部分内容阅读
随着现代科技的发展,实验动物在多方面起着越来越重要的作用。2012年10月,本实验室参与了北京市实验动物管理办公室组织的北京市啮齿类实验动物健康状况调查。采集心、肝、脾、肺、肾、大肠和小肠进行组织病理学观察,通过石蜡切片HE染色、冰冻切片油红O染色、PAS染色等方法对啮齿类实验动物的健康状况进行鉴定。结果显示,部分动物肝脏表现出不同程度的肝细胞肿胀、轻度肺脏病变,少数表现出肾脏管型、肾小囊腔蛋白样物质渗出和大肠寄生虫感染,但是啮齿类实验动物总体水平属健康状况。以上病变的出现可能与动物的生存环境、饲料营养成分和运输处死过程中的应激有关。
With the development of modern science and technology, laboratory animals play an increasingly important role in many aspects. In October 2012, the laboratory participated in the investigation of the health status of rodents in Beijing organized by Beijing Laboratory Animal Management Office. The heart, liver, spleen, lung, kidney, large intestine and small intestine were collected for histopathological observation. HE staining of paraffin sections, oil red O staining of frozen section and PAS staining were used to identify the health status of rodents. The results showed that some animal liver showed varying degrees of hepatocellular swelling and mild lung lesions, and a few showed renal tubular, renal cytoplasmic protein exudation and colorectal parasitic infections, but the overall level of rodent laboratory animals Health status. The occurrence of the above lesions may be related to the living environment of animals, the nutritional content of feed and the stress in the process of transportation and death.