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目的了解分离自四川省巴塘县的首株鼠疫菌的生态型和分子生物学特征,为因地制宜制定鼠疫防制策略提供科学依据。方法将传统的鼠疫菌生化表型鉴定方法和多位点可变数目串联重复序列分析(MLVA)的分型方法相结合,确定巴塘县鼠疫菌株的型别,并进行溯源分析。结果四川省巴塘县鼠疫菌株能够酵解甘油、阿拉伯糖和麦芽糖,且具有硝酸盐还原能力,但不能酵解鼠李糖,符合青藏高原生态型鼠疫菌的表型特征;MLVA型别与分离自青海省和西藏自治区的青藏高原生态型鼠疫菌相同。结论四川省巴塘县鼠疫菌的生态型为青藏高原型,与该菌株亲缘关系最近的鼠疫菌在青海省和西藏自治区均有分布。巴塘县是一个新发现的青藏高原喜马拉雅旱獭型鼠疫自然疫源地。
Objective To understand the ecological and molecular biological characteristics of the first strain of Yersinia pestis isolated from Batang County, Sichuan Province, and to provide a scientific basis for formulating strategies for preventing plague. Methods The traditional Yersinia pestis biochemical phenotyping method was combined with the multi-site variable number tandem repeat analysis (MLVA) typing method to determine the type of the Yersinia pestis strains in Batang County and to make a tracing analysis. Results Rat strains of Batten in Batang County, Sichuan Province were able to digest glycerol, arabinose and maltose, and had the ability of reducing nitrate but not rhamnose, which accorded with the phenotypic characteristics of the Y. pestis in the Qinghai-Tibet Plateau. MLVA type and separation From Qinghai Province and Tibet Autonomous Region of the Tibetan Plateau ecotype Yersinia same. Conclusion The ecotype of Yersinia pestis in Batang County, Sichuan Province is of the Qinghai-Tibetan Plateau type. Both Yersinia pestis strains closely related to this strain are distributed in Qinghai and Tibet Autonomous Region. Batang County is a newly discovered natural foci of the Hormiatus type of plague in the Qinghai-Tibet Plateau.