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目的 建立较为合理的中药虎杖的质量评价标准。方法 化学定性鉴别采用TLC法 ,含量测定采用HPLC法。结果 以白藜芦醇苷和大黄素为对照品 ,以不同的展开系统展开 ,建立了虎杖的TLC定性鉴别方法 ;以白藜芦醇苷为对照品建立了HPLC含量测定方法。结论 建立的TLC方法对虎杖药材的鉴别具有较好的专属性 ,能够区别虎杖与同科属常用药材(2 0 0 0年版中国药典收载的同科根与根茎类药材 )。本文建立的测定白藜芦醇苷含量的HPLC法简单、准确 ,重现性好
Objective To establish a more reasonable quality evaluation standard for Polygonum cuspidatum. Methods TLC method was used for qualitative identification of the chemical and HPLC method was used for content determination. RESULTS: Based on resveratrol glucoside and emodin as the reference products, different development systems were used to establish the TLC qualitative identification method of Polygonum cuspidatum. HPLC method was used to determine the content of resveratrol glucoside. Conclusion The established TLC method has better specificity for the identification of Polygonum cuspidatum, and can distinguish the common medicinal materials used in Polygonum cuspidatum and homologous genus (the same root and rhizome medicines included in the Chinese Pharmacopoeia 2000). The HPLC method for determining the content of polydatin in this article is simple, accurate and reproducible.