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目的:探讨急性冠脉综合征(ACS)患者外周血Foxp3+(包括CD4+Foxp3+和CD4+CD25+Foxp3+)调节性T细胞(Treg)的变化与意义。方法:分别采用流式细胞术(FCM)、实时定量PCR和酶联免疫吸附法(ELISA)检测44例ACS患者,20例稳定性心绞痛(SA)患者和24例对照组患者外周血Treg细胞百分率,转录因子Foxp3的mRNA表达和血浆TGF-β1的浓度。结果:与对照组患者和SA组患者相比,ACS组患者外周血中CD4+Foxp3+、CD4+CD25+Foxp3+Treg细胞的百分率,Foxp3 mRNA的表达和血浆TGF-β1浓度明显降低(P<0.05),而CD4+CD25+Treg细胞的百分率在三组之间并无显著性差异。结论:ACS患者外周血Foxp3+Treg数量和/或功能的下调,Foxp3+Treg的变化可能与斑块的不稳定密切相关。
Objective: To investigate the changes and significance of Foxp3 + (including CD4 + Foxp3 + and CD4 + CD25 + Foxp3 +) regulatory T cells in peripheral blood of patients with acute coronary syndrome (ACS). Methods: The percentage of Treg cells in 44 patients with ACS, 20 patients with stable angina pectoris (SA) and 24 controls were detected by flow cytometry (FCM), real-time quantitative PCR and enzyme-linked immunosorbent assay (ELISA) , MRNA expression of transcription factor Foxp3 and plasma TGF-β1 concentration. Results: Compared with control group and SA group, the percentage of CD4 + Foxp3 +, CD4 + CD25 + Foxp3 + Treg cells, Foxp3 mRNA and plasma TGF-β1 in ACS group were significantly decreased (P <0.05 ), While the percentage of CD4 + CD25 + Treg cells did not differ significantly among the three groups. Conclusion: The decrease of the number and / or function of Foxp3 + Treg in peripheral blood of patients with ACS may be related to the instability of plaque.