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目的:研究中药复方对大鼠冠状动脉结扎后再灌注心肌损伤的保护作用。方法:采用结扎左冠状动脉的方法制备心肌缺血再灌注损伤的动物模型。75只W istar大鼠随机分为假手术组、模型组、中药复方高剂量组、中药复方低剂量组、复方丹参滴丸对照组。中药复方由人参、黄芪、当归、桃仁、红花、赤芍、川芎、檀香、砂仁、丹参、五味子、麦冬等药组成。以血清肌酸激酶(CK)、乳酸脱氢酶(LDH)、丙二醛(M DA)含量和超氧化物歧化酶(SOD)活性,以及心肌组织中内皮素(ET)、降钙素基因相关肽(CGRP)、N a+K+ATP酶(N a+K+ATPase),C a2+ATP酶(C a2+ATPase)活性作为观察指标。结果:中药复方组显著降低了冠状动脉结扎大鼠血清中CK、LDH、M DA含量(P<0.05或P<0.01)与心肌细胞ET活性(P<0.05),提高了SOD、CGRP、N a+K+ATPase、C a2+ATPase活性(P均<0.05),且高剂量组明显优于复方丹参滴丸对照组和中药复方低剂量组(P均<0.05)。结论:中药复方对大鼠冠状动脉结扎后再灌注心肌损伤具有明显的保护作用,其机制可能与改善心肌缺血再灌注冠状微循环与抗氧自由基生成、减轻钙超载和调整血管内皮细胞功能等多种机制有关。
OBJECTIVE: To study the protective effect of traditional Chinese medicine compound on myocardial injury after reperfusion of coronary artery in rats. METHODS: Animal models of myocardial ischemia-reperfusion injury were prepared by ligating the left coronary artery. 75 Wastar rats were randomly divided into sham operation group, model group, high-dose group of traditional Chinese medicine compound, low-dose group of traditional Chinese medicine compound, and compound Danshen dripping pill control group. The traditional Chinese medicine compound consists of ginseng, astragalus, angelica, peach kernel, safflower, red peony, Chuanxiong, sandalwood, Amomum villosum, salvia miltiorrhiza, Schisandra chinensis, and Ophiopogon japonicus. Serum creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA) content and superoxide dismutase (SOD) activity, and endothelin (ET) and calcitonin genes in cardiac muscle tissue The related peptide (CGRP), Na+K+ATPase (N a+K+ ATPase), and Ca a ATPase (C a ATPase) activity were used as observation indexes. RESULTS: The Chinese herbal compound group significantly reduced the serum levels of CK, LDH, MDA (P<0.05 or P<0.01) and myocardial ET activity (P<0.05), and increased SOD, CGRP, Na levels in coronary artery ligation rats. The activities of +K+ATPase and Ca2+ATPase (P<0.05) were significantly higher in the high-dose group than in the compound Danshen Dripping Pills control group and the traditional Chinese medicine compound low-dose group (P<0.05). Conclusion: Traditional Chinese medicine compound has obvious protective effect on myocardial injury after coronary artery ligation in rats, and its mechanism may be to improve coronary microcirculation and antioxidant free radical production in myocardial ischemia-reperfusion, reduce calcium overload and regulate vascular endothelial cell function. And other mechanisms.