人类精子经过卵黄贮存液在4℃状态下长时间获能(至少24小时)后,与去透明带金黄地鼠卵孵化0.5～5小时,分别观察并动态测定其穿透率和受精指数。结果显示,经卵黄贮存液获能后的精子穿透率和受精指数随孵化时间的延长而增高,穿透率以3小时为最高,之后维持在一个较平稳的高水平上,与未经卵黄贮存液获能的精子5小时的穿透率和受精指数间存在显著差异。 Human sperm were incubated with the golden hamster ovum for 0.5 to 5 hours after the egg was stored at 4 ℃ for at least 24 hours. The penetration rate and fertilization index were observed and measured dynamically. The results showed that the sperm penetration rate and fertilization index increased with the incubation time after the egg yolk storage solution was able to be activated, the penetration rate was the highest in 3 hours, then maintained at a relatively stable high level, and without yolk There was a significant difference between the 5-hour penetration rate and the fertilization index of sperm in the stock solution.