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为研究NaAsO_2(亚砷酸钠)中毒对成年SD大鼠纹状体的神经细胞及肝脏形态学的影响,以及NaAsO_2中毒对SD大鼠神经毒性和肝毒性的作用,我们对染毒后的组织进行HE染色并观察。采用12周龄SD大鼠48只,体重200±20 g,随机分组:正常对照组(自由饮水),实验组按剂量递增方式分为低、中、高剂量NaAsO_2染毒组(按照5、10、20 mg/kg的比例配制蒸馏水,自由饮用),各组12只,均采用普通饲料喂养,建造染砷模型12周后取大鼠脑组织纹状体部分,常规石蜡切片后,HE染色,光镜观察大鼠纹状体神经细胞与肝结构的形态学是否发生改变,同时使用试剂盒检测纹状体与肝组织中超氧化物歧化酶(superoxide dismutase,SOD)活力(WST-1法)、还原型谷胱甘肽(reduced glutathione,GSH)(微板法)及丙二醛(malondialdehyde,MDA)(TBA法)的含量。结果显示,利用苏木素-伊红(HE)染色可见:NaAsO_2染毒组的纹状体神经细胞与对照组相比较,神经细胞胞体形态欠规则,数量稀疏且减少,胞浆内可见明显空泡样变,镜下可见坏死水肿的神经细胞。NaAsO_2染毒组的肝组织较正常组出现病理性改变:例如变性、坏死、组织出现水肿及炎性细胞浸润等,与对照组相比,各剂量NaAsO_2染毒组大鼠的纹状体组织及肝脏的SOD活力,GSH含量均降低,差异具有统计学意义(P<0.05);与对照组比较,各剂量NaAsO_2染毒组大鼠的纹状体组织及肝脏的MDA含量均升高,差异具有统计学意义(P<0.05)。由此可知,大鼠纹状体神经细胞形态结构及肝组织结构产生损伤可能由NaAsO_2导致其中毒,同时提示NaAsO_2致大鼠纹状体及肝组织的毒性作用可能与氧化应激相关。
In order to study the effects of NaAsO_2 (arsenite poisoning) on neuronal and hepatic morphology in striatum of adult SD rats and the effect of NaAsO_2 on neurotoxicity and hepatotoxicity in SD rats, HE staining and observation. Forty-eight SD rats of 12 weeks old weighing 200 ± 20 g were randomly divided into normal control group (free drinking water) and experimental group were divided into low, medium and high doses of NaAsO 2 (according to 5,10 , 20 mg / kg ratio of distilled water, free to drink), each group of 12, were fed with ordinary feed, the construction of arsenic poisoning model 12 weeks after taking the rat brain tissue part of the normal paraffin sections, HE staining, The morphological changes of the striatum neurons and the liver were observed under light microscope. The activity of superoxide dismutase (SOD) in striatum and liver tissue was detected by kit (WST-1 method) Reduced glutathione (GSH) and malondialdehyde (MDA) (TBA method). The results showed that the hematoxylin-eosin (HE) staining showed that the neuronal cells in the NaAsO 2 -treated group were less regular and sparsely and less than those in the control group, with obvious vacuolation in the cytoplasm Change, microscope necrotic edema of nerve cells. Compared with the control group, the pathological changes in the NaAsO 2 -treated group were more than those in the normal group, such as degeneration and necrosis, edema and infiltration of inflammatory cells in the NaAsO 2 -treated group, (P <0.05). Compared with the control group, the content of MDA in the striatum and the liver of NaAsO 2 -treated rats increased, the differences were statistically significant Statistical significance (P <0.05). It can be seen that the damage of morphology and structure of rat striatum nerve cells may be caused by NaAsO 2, and the toxic effects of NaAsO 2 on striatum and liver tissue may be related to oxidative stress.