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为了探讨胰岛素水平与消化道溃疡的关系，将成年雄性Ｗｉｓｔａｒ大鼠制成胃溃疡动物模型，用地高辛标记的ｐｒｏ－ＩｎｓｃＲＮＡ探针原位杂交方法，观察大鼠实验性胃溃疡自愈期间胰岛Ｂ细胞内ｐｒｏ－ＩｎｓｍＲＮＡ的变化，并对其杂交信号进行定量分析。结果显示，在胃溃疡模型术后第６和第１０ｄ，胰岛Ｂ细胞内的杂交信号增强，表明在溃疡愈合早期ｐｒｏ－ＩｎｓｍＲＮＡ的表达量增加。结合本室以往的研究结果，初步讨论了胰岛素参与胃溃疡自愈的调节过程及其意义
In order to investigate the relationship between insulin levels and peptic ulcer, adult male Wistar rats were made into animal models of gastric ulcer. Using digoxigenin-labeled pro-InscRNA probe in situ hybridization method to observe the experimental rat gastric ulcer during healing B cell pro-Ins mRNA changes, and its hybridization signal quantitative analysis. The results showed that the hybridization signal of islet B cells increased on the 6th and the 10th day after operation in gastric ulcer model, indicating that pro-Ins mRNA expression increased in the early stage of ulcer healing. Combined with our previous research results, we initially discussed the regulatory process of insulin involved in gastric ulcer healing and its significance