基于Charleston×东农594重组自交系群体,采用完备区间作图和混合线性模型对2006-2010年连续5年的百粒重QTL进行定位,并进行基因×环境互作及上位性分析。结果定位了16个与大豆百粒重性状相关的QTL,其中有5个QTL分别与环境发生互作,互作贡献率在0.11%～0.52%之间;定位了8对上位互作位点,贡献率在1.15%～2.59%之间。 Based on the population of Charleston × Dongnong 594 recombinant inbred lines, the 100-grain weight QTLs for five years in a row from 2006 to 2010 were mapped by complete interval mapping and mixed linear models, and gene × environment interactions and epistasis were analyzed. Results Sixteen QTLs related to 100-grain weight traits were mapped. Among them, 5 QTLs interacted with the environment respectively, with the contribution rates of 0.11% ~ 0.52%. Eight pairs of epistatic sites were located, The contribution rate is between 1.15% ~ 2.59%.