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目的进一步对生物技术及近红外光谱技术在中药鉴别及分析中的应用进行分析和探讨。方法选取东北野生人参以及人工养殖的人参各1批(200条)作为本次的研究对象,分别采用生物技术、近红外光谱技术以及蛋白质指纹图谱分子对两种不同的人参进行鉴别,并利用常规高效液相色谱法进行对比,将结果进行比较。结果两批人参通过不同的方法鉴别后,生物技术对东北野生人参以及人工养殖的人参的鉴别准确率分别为80%、82%,近红外光谱技术对人参的鉴别准确率分别为72%、74%,蛋白质指纹图谱分子对人参的鉴别准确率分别为76%、80%,常规高效液相色谱法对人参的鉴别准确率分别为40%、42%,四组比较差异具有统计学意义(P<0.05)。结论相比于常规高效液相色谱法,生物技术及近红外光谱技术的鉴别准确率更高,操作较为灵活,为从事光谱及中药研究人员的工作提供了诸多便利,为中药的鉴别和分析提供了良好的发展平台。
Objective To further analyze and discuss the application of biotechnology and near infrared spectroscopy in the identification and analysis of traditional Chinese medicine. Methods A total of 200 ginseng (wild ginseng and artificially cultured ginseng from northeastern China) were selected as the research object. Two kinds of ginseng were identified by biotechnology, near infrared spectroscopy and protein fingerprinting respectively. HPLC comparison, the results were compared. Results After the two groups of ginseng were identified by different methods, the identification accuracy of biotechnology to wild ginseng and artificial ginseng in Northeast China were 80% and 82%, respectively, and the accuracy of NIRS was 72% and 74%, respectively %, Respectively. The accuracy of identification of protein fingerprinting molecules on ginseng was 76% and 80%, respectively. The accuracy of routine HPLC for ginseng identification was 40% and 42%, respectively, and the difference between the four groups was statistically significant (P <0.05). Conclusion Compared with conventional high performance liquid chromatography, biotechnology and near-infrared spectroscopy have higher accuracy and flexibility in operation and provide many conveniences for spectrum and traditional Chinese medicine researchers to provide identification and analysis of traditional Chinese medicines A good platform for development.