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AIM: To study the effect of the inhibitor of cytochrome P450 known as Lilly 18947 (2,4 dichloro-6 phenyl-phenoxy ethyl diethylamine) on Trypanosoma cruzi. METHODS: Trypanosoma cruzi epimastigotes wen grown in culture, in absence or in presence of drug. The inhibition of its growth was followed by daily counting using a Neubauer chamber. The effect of Lilly 18947 on the parasite ultrastructure was examined by electron microscopy. To test the effect of different concentrations of drug on the parasite cycle, Vero cells were inoculated with trypomastigotes ( RA strain ) and after 72 h the percentage of infected cells and the number of intracellular parasites were estimated and expressed as the endocytic index. RESULTS: Growth of epimastigotes was inhibited by Lilly 18947. Concentrations as low as 50 μmol/L resulted in a complete disappearance of the parasites in culture by the fourth day. With lower concentrations, little growth was observed and total (25 μmol/L) or partial lysis (10μmol/L) were register
AIM: To study the effect of the inhibitor of cytochrome P450 known as Lilly 18947 (2,4 dichloro-6 phenyl-phenoxy ethyl diethylamine) on Trypanosoma cruzi. METHODS: Trypanosoma cruzi epimastigotes wen grown in culture, in absence or in presence of drug The inhibition of its growth was followed by daily counting using a Neubauer chamber. The effect of Lilly 18947 on the parasite ultrastructure was examined by electron microscopy. To test the effect of different concentrations of drug on the parasite cycle, Vero cells were inoculated with RESULTS: Growth of epimastigotes was inhibited by Lilly 18947. Concentrations as low as 50 μmol / L resulted in a complete disappearance of the parasites in culture by the fourth day. With lower concentrations, little growth was observed and total (25 μmol / L) or partial lysis (10 μmo l / L) were register