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目的研究两个miniSTR复合扩增体系在中国汉族人群中的特征,其中NC01(D10S1248、D14S1434和D22S1045)由欧洲DNA分析工作组推荐,另一体系中D2S2944、D18S872和D19S591位点为自行研究发现。方法利用荧光复合扩增技术进行扩增,采用ABI PRISM 310基因分析仪对产物进行检测,GeneScan 3.7及GenoTyper 3.7软件分析结果。结果所有基因座复合Hardy-Weinberg平衡,6个基因座的累积个人识别率为0.9999,累积非父排除率为0.9793。同时对NC01在中国人群和其他人群中的遗传差异进行了比较。结论建立了两个miniSTR基因座的荧光标记复合扩增体系,这些体系在不同的人群中具有遗传差异。
Objective To study the characteristics of two miniSTR multiplex amplification systems in Chinese Han population. Among them, NC01 (D10S1248, D14S1434 and D22S1045) were recommended by the European DNA Analysis Working Group, and D2S2944, D18S872 and D19S591 in the other system were found on their own. Methods The amplified products were amplified by fluorescent multiplex amplification. The products were detected by ABI PRISM 310 Gene Analyzer and analyzed by GeneScan 3.7 and GenoTyper 3.7 software. Results Hardy-Weinberg equilibrium was found in all the loci. The cumulative personal recognition rate of 6 loci was 0.9999, and the cumulative non-parent exclusion rate was 0.9793. At the same time, the genetic differences between NC01 and other populations in China were compared. Conclusion The fluorescent labeled multiplex amplification system of two miniSTR loci was established. These systems have genetic differences in different populations.