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目的 探讨组织胺及其 1型受体 (H1R)阻断剂、2型受体 (H2R)阻断剂对贮脂细胞收缩的影响。方法 采用肝脏离体胶原酶灌注消化及密度梯度离心的方法来分离培养贮脂细胞 ;用二甲基多聚硅烷烧制聚硅酮膜 ;传代后的贮脂细胞在聚硅酮膜上培养 3d后 ,随机分为 5组 :A组(对照组 ) ;B组 (组织胺 1× 10 -7mol/L组 ) ;C组 (组织胺 1× 10 -6mol/L组 ) ;D组 (H 1R阻断剂 +组织胺 1× 10 -6mol/L组 )和E组 (H2R阻断剂 +组织胺 1× 10 -6mol/L组 )。各组于加药前及加药后 2 0min摄相 ,在相片上分析同一视野细胞周围的聚硅酮膜皱纹变化 ,皱纹增多表明细胞收缩。结果 B组、C组的贮脂细胞收缩率分别为 2 1.0 %、34.2 % ,远高于A组的 3 .8% ,并呈量效依赖关系 (P <0 .0 0 1) ;D组的贮脂细胞收缩率为 17.7% ,低于C组 (P <0 .0 5 ) ;E组的贮脂细胞收缩率为2 6 .3 % ,与C组相比 ,差异无显著性 (P >0 .0 5 )。结论 组织胺通过H1R的介导促进贮脂细胞的收缩 ,可能在门静脉高压症的发生发展中起了一定的作
Objective To investigate the effects of histamine and its receptor type 1 receptor (H1R) and type 2 receptor (H2R) antagonists on the contractile activity of lipid-storing cells. METHODS: Liver-derived collagenase was used for perfusion digestion and density gradient centrifugation to separate and culture the lipid-storing cells. Polysilicone membrane was prepared by using dimethylpolysilane. The passaged fat-storing cells were cultured on the silicone membrane for 3 days Group B (histamine 1 × 10 -7 mol / L), Group C (histamine 1 × 10 -6 mol / L), Group D (H 1R Blocking agent + histamine 1 × 10 -6 mol / L group) and group E (H2R blocking agent + histamine 1 × 10 -6 mol / L group). Before and after dosing, the groups were taken at 20 min, and the change of the wrinkle of the silicone film around the same visual field was analyzed on the photograph. The increase of the wrinkles showed that the cells were contracted. Results The shrinkage rate of fat storage cells in groups B and C were 2.03% and 34.2%, respectively, which was much higher than that in group A (P <0.01) (P <0.05). The shrinkage rate of fat storage cells in group E was 26.3%, which was not significantly different from that in group C (P > 0 .0 5). Conclusions Histamine can promote the contraction of lipid-storing cells through the mediation of H1R, which may play a certain role in the occurrence and development of portal hypertension