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目的:应用模式动物秀丽隐杆线虫研究菊酯类农药氯氰菊酯的生殖毒性,并对秀丽隐杆线虫模型在生殖毒理学中应用的可行性进行初步探讨。方法:将L4期野生型秀丽隐杆线虫分别于0.08、0.8和8.0 mg/L的氯氰菊酯中暴露24、48和72 h,每组10条,以K溶液为阴性对照,分别在体视显微镜和光镜下计数后代数目、子宫内受精卵数目、卵母细胞数目并观察形态,体外排受精卵速率。结果:L4期野生型秀丽隐杆线虫暴露于氯氰菊酯24 h后,各观察终点均无显著性变化;暴露48 h后,8.0 m/L浓度染毒组发生后代数目降低,0.8和8.0 m/L染毒组纳精囊旁侧第2位卵母细胞相对长度减小且子宫内受精卵数目降低,0.08、0.8和8.0mg/L染毒组体外排受精卵速率均下降,与对照组相比,差异均具有统计学意义(P<0.05);暴露72 h后,0.8和8.0 m/L染毒组后代数目降低,纳精囊旁侧第2位卵母细胞相对长度减小,与对照组相比,差异均具有统计学意义(P<0.05)。结论:模式动物秀丽隐杆线虫生殖系统对氯氰菊酯具有一定敏感性,在生殖毒理学研究中具有潜在应用价值。
OBJECTIVE: To study the reproductive toxicity of pyrethroid insecticide cypermethrin using the model animal Caenorhabditis elegans, and to explore the feasibility of using Caenorhabditis elegans model in reproductive toxicology. Methods: The L4 wild-type C. elegans were exposed to cypermethrin at 0.08, 0.8 and 8.0 mg / L respectively for 24, 48 and 72 h, with 10 in each group. K solution was used as negative control. Count the number of offspring under microscope, the number of intrauterine fertilized eggs, the number of oocytes and observe the morphology, in vitro fertilization rate of ovulation. Results: After exposed to cypermethrin for 24 h, L4 wild-type Caenorhabditis elegans showed no significant change at the end of each observation period. After exposure for 48 h, the number of offspring in the 8.0 m / L exposure group decreased 0.8, 8.0 and 8.0 m / L The relative length of the second oocyte in the side of the seminal vesicle of the exposure group was decreased and the number of the zygotes in the uterus was decreased. The rates of in vitro fertilization of ovum in the groups of 0.08, 0.8 and 8.0 mg / L were decreased compared with the control group, (P <0.05). After exposure for 72 h, the number of offspring of 0.8 and 8.0 m / L exposure groups decreased, and the relative length of the second oocyte beside the spermatheca decreased compared with the control group , The differences were statistically significant (P <0.05). Conclusion: The reproductive system of C. elegans in model animals is sensitive to cypermethrin and has potential value in reproductive toxicology research.