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目的研究白藜芦醇对蛋氨酸胆碱缺乏(methionine-choline-deficient,MCD)培养基诱导的小鼠正常肝细胞(alpha monse liver 12,AML12)损伤的保护作用。方法体外培养小鼠正常肝细胞AML12,建立蛋氨酸胆碱缺乏培养基诱导的肝细胞损伤模型,用不同浓度白藜芦醇(25、50、100μmol/L)进行干预24h,检测细胞的增值活性,以及细胞培养基上清液中谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱基酶(LDH)活性和细胞中丙二醛(MDA)水平、甘油三酯(TG)含量,检测细胞中炎性细胞因子IL-1β、IL-6、TNF-α水平及其m RNA水平,并检测微管相关蛋白1轻链3(MAP1-LC3)蛋白的表达。结果与MCD损伤组相比,各白藜芦醇干预组细胞活力明显增强,白藜芦醇可以显著抑制MCD导致的肝细胞ALT,AST,LDH的释放,降低肝细胞内MDA水平和TG含量,降低炎性细胞因子水平,上调MAP1-LC3的表达。结论白藜芦醇对MCD诱导的的小鼠肝AML12细胞损伤有明显的保护作用,自噬可能在其中起到一定的作用。
Objective To investigate the protective effect of resveratrol on the damage of mouse normal hepatocytes (AML12) induced by methionine-choline-deficient (MCD) medium. Methods AML12 cells were cultured in vitro. The model of hepatocellular injury induced by choline chloride deficiency medium was established. The cells were treated with different concentrations of resveratrol (25, 50 and 100 μmol / L) for 24h, (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) activity and malondialdehyde (MDA) levels in the cell culture supernatant, triglyceride (TG) The levels of IL-1β, IL-6, TNF-α and m RNA were detected by ELISA, and the expression of MAP1-LC3 protein was detected. Results Compared with MCD group, resveratrol significantly increased cell viability. Resveratrol significantly inhibited the release of ALT, AST and LDH from hepatocytes induced by MCD, decreased the levels of MDA and TG in hepatocytes, Reduce the levels of inflammatory cytokines and up-regulate the expression of MAP1-LC3. Conclusion Resveratrol has a significant protective effect on MCD-induced injury of mouse liver AML12 cells, and autophagy may play a role in it.