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目的探讨血液甲胎球蛋白信使核糖核酸（ａｌｐｈａｆｅｔａｌｐｒｏｔｅｉｎｍｅｓｅｎｇｅｒＲＮＡ，ＡＦＰｍＲＮＡ）阳性肝癌患者与术后复发或转移的联系。方法应用巢式逆转录多聚酶链反应（ｎｅｓｔｅｄｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ，ＲＴＰＣＲ）检测术后２４小时，４８小时及１周肝癌患者周围静脉血液中ＡＦＰｍＲＮＡ，并随访１～２年。结果术前及术后１周内血液ＡＦＰｍＲＮＡ持续阳性的肝癌患者１２例中，术后１年内出现复发或转移性癌灶６例，术后２年内复发或转移９例；而随访的血液ＡＦＰｍＲＮＡ阴性的肝癌患者１９例中仅有５例在术后１年内复发，在２年内复发８例，无肝外远处器官转移（１年时，二者差异无显著意义，Ｐ＞００５；２年时，二者差异有显著意义，Ｐ＜００５）。结论术后血液ＡＦＰｍＲＮＡ持续阳性的肝癌患者更容易复发或产生远处转移。
Objective To explore the relationship between blood-alfetoprotein messenger RNA (AFPmRNA) positive liver cancer patients and postoperative recurrence or metastasis. Methods Nested polymerase chain reaction (RT-PCR) was used to detect AFP mRNA in peripheral venous blood of patients with HCC at 24 hours, 48 hours and 1 week after operation, and followed up for 1 to 2 years. RESULTS: Of the 12 patients with hepatocellular carcinoma with persistent positive AFP mRNA in the preoperative and postoperative one week, 6 cases had recurrence or metastatic cancer within 1 year after operation, and 9 cases had recurrence or metastasis within 2 years after operation. The follow-up blood AFP mRNA was negative. Of the 19 patients with hepatocellular carcinoma, only 5 recurred within 1 year after surgery, 8 relapsed within 2 years, and no extrahepatic distant organ metastasis (1 year, the difference between the two was not significant, P>005; 2 At the time of year, the difference between the two was significant, P<005). Conclusion Liver cancer patients with persistent positive AFP mRNA in the blood are more likely to relapse or have distant metastases.