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用10和15μg/ml的香烟烟雾凝聚物处理人胚肾原代细胞24小时,一个月左右细胞开始发生转化。转化细胞的超微结构出现一些特征性的改变:细胞的染色体数目变化明显;通过H~3-TdR的掺入量来看,DNA合成旺盛;细胞能在半固体琼脂上生长,目前体外传代培养巳在4年以上,即已获得不死性。
Human embryonic kidney primary cells were treated with 10 and 15 μg / ml of cigarette smoke condensate for 24 hours, and the cells began to undergo transformation about a month later. There are some characteristic changes in the ultrastructure of transformed cells: the number of chromosome changes significantly; by H ~ 3-TdR incorporation, DNA synthesis exuberant; cells can grow on semi-solid agar, the current in vitro culture Has been in 4 years or more, that is, has been undead.