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为了明确不同十字花科蔬菜挥发物对寄生蜂搜索寄主行为的影响,在室内利用“Y”型嗅觉仪测定了卷蛾分索赤眼蜂Trichogrammatoidea bactraeNagaraja对小菜蛾Plutella xylostellaL.常见的6种寄主植物(甘蓝Brassica oleracea、芥蓝Brassica alboglabra、芥菜Brassica juncea、菜心Brassica parachinensis、萝卜Raphanus sativus和西洋菜Nasturtium officinale)及4个不同甘蓝品种的嗅觉行为反应,并用GC-MS对6种健康寄主植物的挥发物进行了化学成分分析。结果表明,卷蛾分索赤眼蜂的嗅觉反应不仅与十字花科蔬菜的种类有关,而且与同一种蔬菜的不同品种有关。6种寄主植物的完整植株对卷蛾分索赤眼蜂雌蜂均具有显著的引诱作用(P<0.05),并且不同种类之间的引诱力大小存在着一定的差异,其中西洋菜对卷蛾分索赤眼蜂的吸引力最强,其次是甘蓝、菜心和芥菜,萝卜和芥蓝的吸引力最差。在测试的4个甘蓝品种完整植株中,雅实绿对卷蛾分索赤眼蜂的引诱力极显著强于日本春夏秋(P<0.01)。GC-MS分析结果显示,6种蔬菜的挥发物中绝大多数是烷烃类物质,其次是烯烃类物质,还有一些醛、醇、酯、酸、酮及噻唑类化合物。其中醛类是西洋菜挥发物中的特有成分,包括5种(2,3-二甲基苯甲醛、辛醛、壬醛、十一醛、十二醛),相对含量总计为4.90%,并且其烷烃类(33种)和醇类(6种)的相对含量分别为47.42%和5.66%,均高于其他几种蔬菜,甘蓝中烯烃类(9种,5.52%)和酸类(4种,12.20%)的种类数和相对含量均为最高。本研究可为生产中蔬菜品种的合理布局和筛选寄生蜂的引诱剂提供理论依据。
In order to clarify the influence of different volatiles of cruciferous vegetables on the host behavior of parasitoids search, 6 species of Plutella xylostella L., a common Trichogrammatoidea bactrae Nagaraja, The host plants (Brassica oleracea, Brassica alboglabra, Brassica juncea, Brassica parachinensis, Raphanus sativus and Nasturtium officinale) and the olfactory behavioral responses of four different cabbage cultivars were analyzed by GC-MS. Plant volatiles were analyzed by chemical composition. The results showed that the olfactory response of Trichogramma decumbens was related not only to the species of cruciferous vegetables but also to the different varieties of the same vegetable. The intact plants of 6 species of host plants had significant inducement on the female Trichogramma nigromaculata (P <0.05), and there was a certain difference in the attractant power among different species. Among them, Trichogramma was the most attractive, followed by cabbage, cabbage and mustard, radish and kale are the most attractive. Among the tested 4 cabbage varieties, the attractant of Aristolochia virginalis was significantly stronger than that of Japanese spring, summer, summer (P <0.01). The results of GC-MS analysis showed that most of the volatiles of the six vegetables were alkanes, followed by olefins, aldehydes, alcohols, esters, acids, ketones and thiazoles. Among them, aldehydes are the special components in the volatile components of Sai Gon and include 5 kinds of (2,3-dimethylbenzaldehyde, octylaldehyde, nonanal, undecylenal and dodecanal) with a relative content of 4.90% The relative contents of alkanes (33 species) and alcohols (6 species) were 47.42% and 5.66%, respectively, higher than those of other vegetables. The relative contents of alkenes (9 species, 5.52% , 12.20%), the number of species and the relative content are the highest. This study can provide a theoretical basis for the reasonable layout of vegetable varieties in production and the attractant for screening parasitoids.