目的初步研究不同剂量壬基酚对雄性大鼠的生精功能及血清激素和睾丸内相关酶的影响。方法雄性大鼠48只,随机分为4组,即对照组、壬基酚低、中、高(50、100、200 mg/kg)剂量组,对照组每日灌胃给予植物油(0.15 ml/100 g),给药组每日灌胃给予壬基酚油溶液,连续28 d。染毒28 d后处死大鼠,取血,放免法检测血清睾酮(T)、皮质酮(CORT)、促卵泡刺激素(FSH)以及黄体生成素(LH)水平。摘取右侧睾丸制备组织匀浆,采用分光光度法测定SOD、MDA、GSH以及LDH的活性。取左侧睾丸分析初级精母细胞染色体畸形率。取附睾,检测附睾尾精子数量、精子活动率和精子畸形率。结果染毒28 d后,与对照组相比,壬基酚组大鼠血清T、FSH和LH水平降低,其中高剂量组降低显著(P<0.05)。CORT升高,但无显著性差异。另外,随染毒剂量增加,大鼠睾丸组织SOD活力、GSH和MDA水平呈现逐渐上升趋势,各染毒组LDH活性升高。壬基酚组大鼠精子数、精子活动率显著低于对照组,精子畸形率显著高于对照组,初级精母细胞染色体畸变率高于对照组。结论壬基酚对雄性大鼠有明显的生殖毒性。其可能通过降低大鼠血清T、FSH和LH水平,增加睾丸组织GSH和LDH活力产生作用。 Objective To study the effects of different doses of nonylphenol on spermatogenic function and serum hormone and testis-related enzymes in male rats. Methods Forty-eight male rats were randomly divided into 4 groups: control group, low, medium and high dose of nonylphenol (50,100,200 mg / kg) 100 g). The administration group was orally administered nonylphenol oil solution orally for 28 consecutive days. Rats were killed after 28 days of exposure and blood and radioimmunoassay were used to detect serum testosterone (T), corticosterone (CORT), follicle stimulating hormone (FSH) and luteinizing hormone (LH). Tissue homogenates were harvested from the right testes and the activities of SOD, MDA, GSH and LDH were determined by spectrophotometry. Left testis analysis of primary spermatocyte chromosome aberration rate. Epididymis was taken, the number of sperms in epididymal tail, sperm motility and sperm deformity were measured. Results Compared with the control group, the levels of serum T, FSH and LH in the nonylphenol group decreased significantly (P <0.05). CORT increased, but no significant difference. In addition, with the increase of exposure dose, SOD activity, GSH and MDA in rat testis showed a gradual upward trend, LDH activity increased in each exposure group. Nonylphenol group sperm count, sperm motility was significantly lower than the control group, sperm deformity was significantly higher than the control group, primary spermatocyte chromosome aberration rate was higher than the control group. Conclusion Nonylphenol has obvious reproductive toxicity to male rats. It may by reducing rat serum T, FSH and LH levels, increased testicular GSH and LDH activity.