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目的研究铝对大鼠脑组织脂质过氧化作用的影响及其性别差异,为进一步探讨铝的神经毒作用机制提供依据。方法将40只SD大鼠分为4组,每组10只,雌雄各半,每组大鼠分别腹腔注射0．0、2．0、4．0、8．0 AL／kg,连续3 d,间隔1 d,共60 d。用跳台法测试其学习记忆能力的改变,用试剂盒测定其脑组织中的超氧化物岐化酶(SOD)活力和丙二醛(MDA)含量的改变,同时采用透射电镜观察其脑组织超微结构的变化。结果各组不同性别大鼠的各项指标的改变之间差异无统计学意义(P>0．05)。与对照组相比,染铝组大鼠的学习记忆能力明显降低,差异有统计学意义(P<0．05),脑组织内SOD活力降低、MDA含量增高,差异有统计学意义(P<0．05)。电镜观察可见染铝组大鼠大脑皮层神经细胞的膜结构破裂、溶解、消失。结论铝可能通过增强大鼠脑组织的脂质过氧化反应而产生神经毒作用,尚不能认为该作用有性别差异。
Objective To study the effect of aluminum on lipid peroxidation in rat brain and its gender differences in order to provide a basis for further exploring the mechanism of aluminum neurotoxicity. Methods Forty Sprague Dawley rats were divided into 4 groups with 10 rats in each group. The rats in each group were injected intraperitoneally with 0.0, 2.0, 4.0 and 8.0 AL / kg for 3 d , An interval of 1 d, a total of 60 d. The changes of learning and memory abilities were measured by the method of step-down test. The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in brain tissue were measured by kit, and the ultrastructure of brain tissue was observed by transmission electron microscope Microstructure changes. Results There was no significant difference in the changes of various indexes between different sex groups in each group (P> 0.05). Compared with the control group, the learning and memory abilities of rats in the aluminum-treated group were significantly decreased (P <0.05), the activity of SOD in the brain decreased and the content of MDA increased (P < 0.05). Electron microscopy showed that the membrane structure of cerebral cortical neurons in aluminum-exposed rats ruptured, dissolved and disappeared. Conclusion Aluminum may cause neurotoxic effects by enhancing lipid peroxidation in rat brain tissue. However, this effect can not be considered as a gender difference.