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目的:研究乳腺癌患者腋下淋巴结细胞诱导产生的肿瘤特异性CTLs在裸鼠体内对患者自身乳腺癌生长的抑制作用。方法:在裸鼠胸垫处种植人乳腺癌组织,建立裸鼠乳腺癌移植瘤模型。以细胞因子联合诱导乳腺癌患者腋窝淋巴结单个核细胞成为DC和肿瘤特异性CTL(sCTL)、非特异性CTL(nCTL)和异体CTL(vCTL);将不同CTLs注射到荷瘤裸鼠的肿瘤周围,观察其对肿瘤生长的抑制作用。结果:人乳腺癌移植瘤在裸鼠体内的成活率为100%。组织病理学证实移植瘤为乳腺浸润性导管癌。与对照组相比,荷瘤裸鼠移植瘤的体积在sCTL、nCTL和vCTL治疗组均明显减小(P<0.05),sCTL对自体移植瘤的生长抑制作用最强[平均瘤体积(82.70±2.09)mm3],抑瘤率50.5%,明显高于nCTL组[(96.15±5.35)mm3,26.9%]和vCTL组[(96.93±4.51)mm3,25.7%],(P<0.01);不同CTL治疗组瘤组织内可见大量淋巴细胞和树突状细胞(DC)浸润。结论:乳腺癌裸鼠移植瘤模型成功率高,病理学证实与人乳腺癌特征相符,且有DC和大量特异性CTL浸润。自体特异性CTL对裸鼠体内自体乳腺癌移植瘤有较强的抑制作用。
OBJECTIVE: To study the inhibitory effect of tumor-specific CTLs induced by underarm lymph node cells in breast cancer patients on their own breast cancer growth in nude mice. Methods: Breast cancer tissue was implanted in nude mouse chest pad to establish a model of breast cancer xenograft in nude mice. The combination of cytokines induced breast cancer patients with axillary lymph node mononuclear cells into DC and tumor-specific CTL (sCTL), non-specific CTL (nCTL) and allogeneic CTL (vCTL); different CTLs were injected into tumor-bearing nude mice around the tumor, Observe its inhibitory effect on tumor growth. Results: The survival rate of human breast cancer xenografts in nude mice was 100%. Histopathology confirmed that the transplanted tumor was invasive breast ductal carcinoma. Compared with the control group, the volume of xenografted tumor in nude mice significantly decreased (P <0.05) in the sCTL, nCTL and vCTL treatment groups, and sCTL had the strongest growth inhibitory effect on the autograft tumor [mean tumor volume (82.70 ± (96.93 ± 4.51) mm3, 25.7%] in vCTL group (P <0.01). The CTL of different CTL A large number of lymphocytes and dendritic cells (DCs) infiltrated in the treated group. Conclusion: The success rate of breast cancer xenograft model in nude mice is high, the pathology confirmed that it is consistent with the characteristics of human breast cancer, and DC and a large number of specific CTL infiltration. Autologous CTL has a strong inhibitory effect on autologous breast cancer xenografts in nude mice.