利用光周期调控技术诱导甘蔗热带种路打士开花,并与滇蔗茅云南95-19进行远缘杂交,培育实生苗76株,大田移栽成活62株,成活率81.6%;选用4对引物对获得的62份杂交后代进行SSR分子标记鉴定,结果表明,62份杂交后代全部为真杂种,所选SSR引物对滇蔗茅后代群体具有较高的鉴定效率。该杂交组合后代杂种真实率为100%,是目前数量最大的滇蔗茅F1群体。 The photoperiod control technology was used to induce the flowering of sugarcane tropical plants and distant hybridization was carried out with Yunnan 95-19, which resulted in 76 seedlings and 62 surviving transplants. The survival rate was 81.6%. Four pairs of primers SSR markers were used to identify the 62 hybrid progenies. The results showed that all the 62 hybrid progenies were true hybrids, and the selected SSR primers had high efficiency in identifying the progenies of D. yunnanensis. The true rate of offspring hybrids is 100%, which is the largest population of Dian Cimicifuga F1 at present.