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采用亚型测定、核苷酸和氨基酸序列测定和同源性分析等方法 ,观察了HIV 1ⅢB毒株在实验室长期传代过程中包膜基因变异的情况。研究的毒株包括 :经过实验室 8年多连续使用而获得的毒株、在MT4细胞长期连续传代而获得的每间隔 10代的毒株样品及多次更换宿主细胞传代而获得的毒株。主要结果有 :(1)各种毒株包膜基因变异均不显著 ,核苷酸序列同源性均大于 92 % ,变异距离均小于 7.5 % ,且随着传代数增加核苷酸趋于稳定 ,代间同源性由 92 %上升至 99% ,而变异距离由 7.5 %下降为 0 .6 %。 (2 )在传代过程中HIV 1亚型保持稳定 ,各种毒株均为HIV 1B3亚型。结果显示HIV在体外长期传代培养的过程中变异不大 ,遗传性状稳定 ,可能是体外生长的环境十分稳定 ,缺乏机体免疫学压力。结果也提示体外长期传代HIV毒株仍然适用于各种HIV的应用研究 ,用长期传代的方法发展HIV 减毒活疫苗的可能性不大
Subtype determination, nucleotide and amino acid sequence determination and homology analysis were used to observe the variation of envelope gene in HIV 1 Ⅲ B strains during long-term passage in the laboratory. Strain strains studied include: strains obtained after continuous use in the laboratory for more than eight years, samples of strains at intervals of 10 generations obtained by long-term continuous passage of MT4 cells, and strains obtained by multiple generations of passage of host cells. The main results are as follows: (1) The variation of envelope genes of various strains was not significant, the nucleotide sequence homology was more than 92% and the variation distance was less than 7.5%, and the nucleotides tended to be stable with the passage number increasing , The homology increased from 92% to 99%, while the variation distance decreased from 7.5% to 0.6%. (2) HIV 1 subtype remained stable during passage, and all strains were HIV 1B3 subtypes. The results showed that HIV in the process of long-term subculture in vitro little variation, genetic stability, may be in vitro growth environment is very stable, lack of immunological pressure on the body. The results also suggest that in vitro long-term passage of HIV strains are still suitable for the application of various HIV research, the long-term generation of HIV live attenuated vaccine development is unlikely