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采用雌雄兼有的成年小鼠40只,分4组分别置入合常氧、12%、8%、4%氧的密闭容器内1O～15min.经心脏取血测动脉血氧分压,血氧饱和度.取心室肌按Nitro-BT法测琥珀酸脱氢酶,以硝酸铅法Ca~(2+)-Mg~(2+)-腺苷三磷酸酶,并通过电镜观察心肌超微结构变化.结果表明,当吸入氧浓度等于或小于8%时,琥珀酸脱氢酶损伤反应明显;等于或小于12%时.Ca~(2+)-Mg~(2+)-腺苷三磷酸酶活性明显降低;当吸入氧浓度为4%时,心肌超微结构出现明显地不可逆性改变.结果提示,当小鼠吸入等于或小于12%的氧15min时,心肌出现明显的代谢障碍.
Forty adult male and female mice were divided into four groups, respectively, into the normal oxygen, 12%, 8%, 4% oxygen in airtight containers 1O ~ 15min. The heart blood was measured arterial oxygen partial pressure, blood Oxygen saturation was measured in ventricular myocardium by Nitro-BT method to measure succinate dehydrogenase, lead nitrate method Ca ~ (2 +) - Mg ~ (2 +) - adenosine triphosphatase and electron microscopy of myocardial ultrastructure (2 +) - Mg ~ (2 +) - adenosine triphosphate at 12% or lower, respectively, when the oxygen concentration was 8% or less, The activity of phosphatase was significantly decreased, and the myocardial ultrastructure was obviously irreversibly changed when the concentration of oxygen inhaled was 4% .The results showed that when the mice inhaled oxygen at 15% or less for 12 min, the myocardial metabolism was obviously.