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目的 探讨乙型肝炎病毒 (HBV)基因疫苗联合乙型肝炎病毒表面抗原S蛋白 (HBsAg)免疫Balb/c小鼠的效应。方法 构建编码S蛋白的重组真核表达质粒pCR3 .1-S作为HBV基因疫苗。以HBV基因疫苗联合HBsAg蛋白免疫接种Balb/c小鼠 ,同时以HBV基因疫苗或S蛋白单独接种Balb/c小鼠作为对照。采用ELISA法检测免疫小鼠的抗HBs应答 ,3 H -TdR掺入法检测免疫小鼠的淋巴细胞增殖反应。结果 免疫接种 2周、4周后 ,联合免疫组抗HBs滴度高于HBV基因疫苗单独接种组 ,但低于S蛋白单独接种组 ;6周后 ,HBV基因疫苗组抗HBs滴度最高 ,联合组次之。各组间免疫小鼠的淋巴细胞增殖反应无显著差异性 ( P >0 .0 5 )。结论 HBV基因疫苗联合S蛋白免疫Balb/c小鼠无优势
Objective To investigate the effect of Hepatitis B virus (HBV) gene vaccine combined with hepatitis B virus surface antigen S protein (HBsAg) on Balb / c mice. Methods Recombinant eukaryotic expression plasmid pCR3.1-S encoding S protein was constructed as HBV gene vaccine. Balb / c mice were immunized with HBV gene vaccine combined with HBsAg protein, while Balb / c mice were inoculated with HBV gene vaccine or S protein separately as a control. The anti-HBs response of the immunized mice was detected by ELISA, and the lymphocyte proliferation reaction of immunized mice was detected by 3 H-TdR incorporation. Results After 2 and 4 weeks of immunization, the titer of anti-HBs in the combination group was higher than that in the HBV group alone but lower than that in the group S alone. After 6 weeks, the titer of anti-HBs in the HBV group was the highest Group second. There was no significant difference in lymphocyte proliferation between immunized mice in each group (P> 0.05). Conclusion HBV gene vaccine combined with S protein immunized Balb / c mice had no advantage