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将包裹了三(2,2’-联二吡啶)二氯化钌(Ⅱ)配合物(Ru(bpy)32+)的二氧化硅纳米粒子(Ru(bpy)32+@Si O2)通过Nafion膜修饰在玻碳电极上,并研究了咖啡因在此电极上的电化学发光行为。在单因素实验的基础上,结合响应面分析法对实验条件进行了优化,建立了一种检测咖啡因的新方法。在最佳的实验条件下,咖啡因浓度在5.0×10-3~20μmol/L范围内与其相对发光强度呈良好的线性关系(r2=0.9996),检出限(S/N=3)为1.3×10-3μmol/L。连续平行测定1μmol/L的咖啡因溶液5次,相对发光强度的RSD为3.8%。方法用于可乐样品的实际测定,回收率为93.7%~103.8%。
Silica nanoparticles (Ru (bpy) 32 + @ Si O2) coated with a tris (2,2’-bipyridyl) ruthenium (II) dichloride complex (Ru (bpy) 32+) were passed through Nafion The membrane was modified on a glassy carbon electrode and the electrochemiluminescence behavior of caffeine on the electrode was investigated. On the basis of single factor experiment, combined with response surface analysis to optimize the experimental conditions, a new method for the determination of caffeine was established. Under the optimal experimental conditions, the concentration of caffeine showed a good linear relationship with the relative luminescence intensity in the range of 5.0 × 10-3 ~ 20μmol / L (r2 = 0.9996) and the detection limit (S / N = 3) was 1.3 × 10-3μmol / L. The results showed that the RSD of the relative luminescence intensity was 3.8% when the 1 μmol / L caffeine solution was measured in parallel and in parallel for 5 times. The method was applied to the actual determination of cola samples with recoveries of 93.7% ~ 103.8%.