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已发现,睾丸局部产生多种肽类,它们似可能在不同的睾丸细胞间起信息传递作用。最近,在人类的多种外周组织中均发现存在促肾上腺皮质激素释放因子(CRF)样活性。此外,已检出在大鼠的外周组织和睾丸上具有 CRF 的结合位点。本文使用放射免疫分析法测出大鼠睾丸具有免疫反应的CRF,并用免疫细胞化学测定观察了 CRF 在睾丸和附睾的定位。选用不同年龄的 SD 大鼠以及于鼠龄60天切除垂体的大鼠进行实验。另于60天的大鼠或行单侧睾丸切除或造成单侧隐睾(手术移植单侧睾丸于腹腔
It has been found that testis locally produces a variety of peptides that appear to act as messengers of transmission between different testicular cells. Recently, corticotropin-releasing factor (CRF) -like activity has been found in many human peripheral tissues. In addition, binding sites with CRF on the peripheral tissues and testes of rats have been detected. In this paper, radioimmunoassay was used to detect CRF in rat testes, and the localization of CRF in testis and epididymis was observed by immunocytochemistry. Selection of different ages of SD rats and rats 60 days after resection of the pituitary rats experiments. The other 60 days in rats or unilateral testicular excision or cause unilateral cryptorchidism (surgical transplantation of unilateral testes in the abdominal cavity