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为进一步明确香蕉叶斑病菌喙突脐蠕孢的生物学特性,调查了部分环境因素(碳氮源、植物成分培养基)对供试菌株(CLER09、D087和JL05)的营养生长和产孢的影响。结果显示,分生孢子主要先从基部萌芽,在28℃下约培养10h后出现两端萌芽;供试菌株对测试的20种碳源及26种氮源显示相似的生长反应,均可利用除菊糖外的测试碳源及氮源进行营养生长和产孢;测试的6种植物成分培养基对供试菌株的营养生长效果皆优于PDA培养基;除燕麦培养基外,其余5种培养基对菌株CLER09和D087的产孢作用均优于PDA培养基;测试的6种培养基对菌株JL05的产孢作用均优于PDA培养基。不同植物成分培养基对该菌分生孢子形态影响较大,以米糠、象草培养基对菌株D087和JL05的分生孢子长度和玉米粉培养基对菌株CLER09的分生孢子宽度的增长效果最为明显。
In order to further clarify the biological characteristics of B. cercariae, we investigated the effects of some environmental factors (carbon and nitrogen sources, plant component medium) on vegetative growth and sporulation of tested strains (CLER09, D087 and JL05) influences. The results showed that the conidia germinated mainly from the base and germinated at about 10 h after culturing at 28 ℃. The tested strains showed similar growth responses to the 20 carbon sources tested and 26 nitrogen sources, Inulin and nitrogen sources were used for vegetative growth and sporulation. The tested 6 kinds of plant component culture medium had better effect on growth of the tested strains than PDA medium. In addition to the oat medium, the other 5 kinds of culture The sporulation of the strains CLER09 and D087 were better than that of PDA. The spores of strain JL05 were better than that of PDA in the six media tested. The different plant component medium had a great influence on the conidial morphology of the strain. The growth of conidia length of strain D087 and JL05 and conidia width of strain CLER09 in rice medium and grass culture medium were the most obvious.