Pathogenic viruses in drinking water are great threats to public health.Visible-light-driven photocatalysis is a promising technology for virus inactivation.However,the existing photocatalytic antiviral research studies have mostly been carried out in single-component systems,neglecting the effect of natural organic matter,which exists widely in actual water bodies.In this paper,electrospun Cu-TiO2 nanofibers were prepared as photocatalysts,and their photocatalytic antiviral performance in the presence of humic acid (HA) was comprehensively studied for the first time.The properties of the reaction mixture were measured during the reaction.In addition,the safety,reliability and stability of photocatalytic disinfection in the mixed system were evaluated.The results showed that the virus removal efficiency decreased with the increase of the HA concentration.The type of reaction solution,such as PBS buffer solution or water,did not affect the removal efficiency noticeably.Under acidic conditions,the electrostatic forces between photocatalysts and viruses were strengthened,leading to higher virus removal efficiency.As the reaction time went on,the pH value in the solution increased first and then tended to be stable,the conductivity remained stable,and the dissolved oxygen increased first and then decreased.The safety test showed that the concentration of Cu ions released into the solution was lower than specified by the intational standards.No photoreactivation was observed,and the addition of HA significantly reduced the reutilization efficiency of the photocatalysts.