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目的探讨碱性成纤维细胞生长因子(bFGF)基因修饰的组织工程化复合物对牙龈组织缺损再生的影响。方法本研究于2007年7月至2009年1月在福建省高校口腔医学重点实验室进行。利用bFGF基因转染Beagle犬牙龈成纤维细胞(GFs),并将其接种于脱细胞真皮基质(ADM)形成组织工程化复合物,植入Beagle犬的人工牙周组织缺损区,通过对治疗前后牙周指标测量分析,评价该组织工程化复合物对牙龈组织再生的影响。结果实验前后附着丧失差值测定显示GFs与ADM复合物组及转染bFGF基因的GFs复合物组之间无显著性差异,与空白对照组相比均有明显增加(P<0.05)。角化龈宽度差值,6周时转染基因组有明显增加,12周时两实验组均比空白对照组明显增加(P<0.05)。结论复合GFs的ADM可能有助于改善附着丧失的程度及角化龈宽度,而bFGF的作用并不肯定。组织工程技术能有效促进牙龈组织再生。
Objective To investigate the effect of basic fibroblast growth factor (bFGF) gene modified tissue engineered composite on the regeneration of gingival tissue defects. Methods The study was performed in Key Laboratory of Stomatology, Fujian University from July 2007 to January 2009. Beagle dog gingival fibroblasts (GFs) were transfected with bFGF gene and inoculated into acellular dermal matrix (ADM) to form tissue engineered complexes. The artificial periodontal defects in Beagle dogs were implanted. Periodontal measurements were measured to assess the effect of the tissue engineered compound on gingival tissue regeneration. Results The difference of adhesion loss before and after the experiment showed that there was no significant difference between the GFs and ADM complex groups and the GFF complex group transfected with bFGF gene, which were significantly increased compared with the blank control group (P <0.05). At the 12th week, the two experimental groups were significantly higher than the blank control group (P <0.05). Conclusions ADM with composite GFs may help to improve the degree of attachment loss and the width of keratinized gingiva, but the effect of bFGF is not certain. Tissue engineering technology can effectively promote the regeneration of gingival tissue.