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目的:考察五味子甲素对小鼠溃疡性结肠炎的治疗作用。方法:以三硝基苯磺酸(TNBS)灌肠制备溃疡性结肠炎(ulcerative colitis,UC)小鼠模型,溃疡性结肠炎小鼠随机分为模型组、阳性对照组及五味子甲素高、中、低剂量组(80、40、20 mg/kg),同时设立正常对照组,每组10只。灌胃给药,正常组、模型组分别给予等体积蒸馏水,阳性对照组给予500 mg/kg柳氮磺吡啶(SASP),连续给药14 d。观察五味子甲素对溃疡性结肠炎小鼠临床症状的影响;酶联免疫吸附法(ELISA)检测溃疡性结肠炎小鼠结肠中肿瘤坏死因子-α(TNF-α)及白介素-6(IL-6)水平;用试剂盒检测结肠中髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)及一氧化氮合成酶(TNOS)的活性和丙二醛(MDA)、一氧化氮(NO)的含量。取结肠后HE染色观察组织形态学变化。结果:五味子甲素可改善三硝基苯磺酸诱导的溃疡性结肠炎小鼠临床症状,延长溃疡性结肠炎小鼠结肠转运时间,可显著降低MPO、TNOS活性,升高SOD活性,并降低MDA、NO、IL-6及TNF-α水平。组织病理学检查可见,给予五味子甲素后,溃疡性结肠炎小鼠病变严重程度减轻,动物结肠组织损伤范围明显减少,炎性细胞浸润程度减轻。结论:五味子甲素对三硝基苯磺酸诱导的小鼠溃疡性结肠炎有治疗作用,作用机制可能与其抑制炎症因子NO、IL-6及TNF-α的产生有关。
Objective: To investigate the therapeutic effect of Schisandrin on mouse ulcerative colitis. Methods: Ulcerative colitis (UC) mouse model was established by TNBS enema. The ulcerative colitis mice were randomly divided into model group, positive control group and schizandrin A high and medium , Low dose group (80, 40, 20 mg / kg), at the same time set up normal control group, 10 in each group. Oral administration, normal group, model group were given equal volume of distilled water, the positive control group was given 500 mg / kg sulfasalazine (SASP), continuous administration of 14 d. To observe the effect of Schisandrin A on the clinical symptoms in mice with ulcerative colitis. The levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the colon of mice with ulcerative colitis were detected by enzyme-linked immunosorbent assay (ELISA) 6). The activities of MPO, SOD and TNOS in colonic mucosa were detected by enzyme-linked immunosorbent assay (ELISA), the activity of malondialdehyde (MDA), nitric oxide NO) content. The morphological changes of the colon were observed by HE staining. Results: Schisandrin could improve the clinical symptoms of mice with ulcerative colitis induced by trinitrobenzene sulfonic acid and prolong the colon transit time in mice with ulcerative colitis, significantly reduce the activity of MPO and TNOS, increase the activity of SOD and decrease MDA, NO, IL-6 and TNF-α levels. Histopathological examination shows that, after administration of Schisandrin A, the lesion severity of ulcerative colitis mice was alleviated, the extent of damage to the colon tissues was significantly reduced, and the infiltration of inflammatory cells was alleviated. CONCLUSION: Schizandrin can treat mice with ulcerative colitis induced by trinitrobenzene sulfonic acid. Its mechanism may be related to the inhibition of the production of NO, IL-6 and TNF-α.