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本文建立了由磷酸氢二钾、磷酸二氢钾、聚乙二醇及水所组成的双水相体系（不含生物物质）的平衡浓度分析测定方法。实验共测定了ｐＨ＝９．４３及聚乙二醇数均分子量分别为１０００，２０００，４０００和６０００四种共４１组在２５℃下的液－液平衡数据；测定了聚乙二醇数均分子量为４０００和ｐＨ值分别为６、７、８时共３２组在２５℃下的液液平衡数据；实验还测定了Ｋ２ＨＰＯ４和ＫＨ２ＰＯ４在水中的缔合常数；用扩展的ＵＮＩＦＡＣ方程（短程项）和Ｆｏｗｌｅｒ－Ｇｕｇｇｅｎｈｅｉｍ方程（长程项）相结合进行了关联和预测，结果满意。同时还用Ｓｅｔｓｃｈｅｎｏｗ公式对双水相中上层贫盐水相进行关联，并发现此双水相体系遵循盐效应的一般规律。
In this paper, a method for the determination and determination of equilibrium concentration of biphasic aqueous system (without biological substances) consisting of dipotassium hydrogen phosphate, potassium dihydrogen phosphate, polyethylene glycol and water has been established. In the experiment, the liquid-liquid equilibrium data of 41 groups with pH = 9.43 and number-average molecular weight of polyethylene glycol of 1000, 2000, 4000 and 6000 at 25 ℃ were measured. The average number of polyethylene glycol Molecular weight of 4000 and pH values of 6, 7, 8, respectively, a total of 32 groups of liquid-liquid equilibrium data at 25 ℃; experiment also determined K2HPO4 and KH2PO4 in water association constants; with extended UNIFAC equation (short term) And Fowler-Guggenheim equation (long-term) combined with the correlation and prediction, the results are satisfactory. At the same time, the formula of Setschenow was used to correlate the upper aqueous salt phase in the double aqueous phase. It was found that the aqueous two-phase system follows the general rule of salt effect.