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以大麦黄矮病毒ＧＰＶ 株系的ＲＮＡ 为模板， 采用反转录－聚合酶链式反应（ＲＴ－ＰＣＲ）方法，扩增出１１３４ｂｐ 的缺失复制酶基因。将扩增的片段克隆到ｐＵＣ１９ 的Ｓｍ ａＩ位点上并进行了序列测定，获得正向插入的重组质粒ｐＵＣ１９Ｄ。以ＫｐｎＩ和ＸｂａＩ从ｐＵＣ１９Ｄ 上切下此基因并插入质粒ｐＥｍ ｕ－ｍ ｃｓ－Ｎ 得到植物表达载体ｐＰＰＩ８。应用花粉管通道法转化普通小麦品种陇鉴１２７、陕１６０ 和晋麦４７，通过卡那霉素抗性筛选、ＰＣＲ和ＰＣＲ－Ｓｏｕｔｈｅｒｎ 检测，３ 个品种均获得了转基因小麦植株。
Using the RNA of GPV strain of barley yellow dwarf virus as a template, the 1134bp deletion replicase gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR). The amplified fragment was cloned into SmII site of pUC19 and sequenced to obtain a recombinant plasmid pUC19D inserted in the forward direction. This gene was excised from pUC19D with KpnI and XbaI and the plasmid pEm u-m cs-N was inserted to obtain the plant expression vector pPPI8. The common wheat varieties Longjian 127, Shaan 160 and Jinmai 47 were transformed by pollen tube pathway. The transgenic wheat plants were obtained from all three cultivars by kanamycin resistance screening, PCR and PCR-Southern detection.