论文部分内容阅读
聚合酶链反应(ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ,ＰＣＲ)技术的原理类似于ＤＮＡ的体内复制,只是在试管中给ＤＮＡ的体外合成提供一种合适的反应条件模板ＤＮＡ、寡板苷酸引物、ＤＮＡ聚合酶、Ｍｇ2+、合适的缓冲体系和ＤＮＡ变性、复性、及延伸的温度与时间等
The principle of polymerase chain reaction (PCR) is similar to the in vivo replication of DNA except that a suitable reaction condition is provided for the in vitro synthesis of DNA in a test tube. Template DNA, oligo-nucleotide primers, DNA polymerase, Mg2 + Suitable buffer systems and DNA denaturation, refolding, and extended temperature and time