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目的探讨碱性成纤维细胞生长因子（ｂＦＧＦ）在人类增殖性肾小球肾炎（ＰＧＮ）中的表达及作用。方法采用原位杂交、免疫组化和放免测定方法检测肾活检组织中的ｂＦＧＦｍＲＮＡ及蛋白质表达、肾小球内细胞外基质（ＥＣＭ）面积和有核细胞数、层粘蛋白（ＬＮ）和前胶原Ⅲ（ＰＣⅢ）含量。结果在以细胞增殖和ＥＣＭ积聚为特征的ＰＧＮ组中ｂＦＧＦ表达水平、ＬＮ和ＰＣⅢ含量均显著高于非增殖性肾小球肾炎（ＮＰＧＮ）组和正常组。结论过度表达的ｂＦＧＦ参与肾小球内细胞增殖和ＥＣＭ积聚，从而影响肾小球肾炎和肾小球硬化进程。
Objective To investigate the expression of basic fibroblast growth factor (bFGF) in human proliferative glomerulonephritis (PGN) and its role. Methods In situ hybridization, immunohistochemistry and radioimmunoassay were used to detect the expression of bFGF mRNA and protein in renal biopsies, the area of glomerular extracellular matrix (ECM) and the number of nucleated cells, laminin (LN) and procollagen Ⅲ (PC Ⅲ) content. Results The expression of bFGF, LN and PCⅢ in PGN group characterized by cell proliferation and ECM accumulation were significantly higher than those in non-proliferative glomerulonephritis (NPGN) group and normal group. Conclusion Overexpression of bFGF is involved in the proliferation of glomerular cells and the accumulation of ECM, thus affecting glomerulonephritis and glomerulosclerosis.