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该实验应用~3H-TdR参入被PHA激活淋巴细胞的方法,当加入5μg/ml VitA培养16h后,发现~3H-TdR参入淋巴细胞计数率(cpm)明显增高;而浓度超过25μg/ml时,或浓度为10μg/ml作用72h时,参入量均明显下降。淋巴细胞经不同剂量的~(60)Coγ射线照射后进行体外培养发现,加入VitA 5μg/ml 16h,~3H-TdR参入DNA的cpm比单纯照射组均有不同程度的提高。10例癌症患者接受一个疗程的放疗(剂量75～184 Gy),其~3H-TdR参入率显著下降,加入5μg/ml的VitA,其~3H-TdR参入率比对照组非常显著增高。以上结果均提示适当浓度VitA具有促进PHA激活淋巴细胞转化的作用,而LPS激活的B细胞同样加入VitA,则不表现促进作用。
In this experiment, ~3H-TdR was used as a method for PHA-activated lymphocytes. When 5 μg/ml VitA was added for 16 hours, the number of ~3H-TdR incorporation lymphocytes (cpm) was significantly increased; when the concentration exceeded 25 μg/ml, When the concentration was 10 μg/ml for 72 h, the amount of incorporation decreased significantly. Lymphocytes were cultured in vitro with different doses of ~(60)Co γ-rays. After addition of VitA 5 μg/ml for 16 h, the cpm of ~3H-TdR incorporation into DNA was increased to a different extent than that of the irradiated group. 10 patients with cancer received a course of radiotherapy (dose 75-184 Gy), their ~3H-TdR participation rate decreased significantly, adding 5μg/ml of VitA, its ~3H-TdR participation rate was significantly higher than the control group. These results suggest that the appropriate concentration of VitA has the effect of promoting the PHA-activated lymphocyte transformation, while the LPS-activated B cells also added VitA, it does not show a promoting effect.