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目的探讨大蒜油减少小鼠正己烷活性代谢产物的生成作用,为减少正己烷中毒提供实验依据。方法健康成年昆明小鼠,随机分为正己烷染毒组和大蒜油干预组,分别灌胃给予正己烷和大蒜油+正己烷,取血,分离血清经乙酸乙酯萃取后,气相色谱法测定血清中2,5-己二酮(2,5-HD)含量。结果小鼠经正己烷单次灌胃染毒后测定血清2,5-HD含量为(0.14±0.14)μg/mL,10 h后达峰值(24.63±3.52)μg/mL,至染毒后20 h降至(0.02±0.04)μg/mL;正己烷染毒前后给予大蒜油使小鼠6h血清2,5-HD含量分别较单纯染毒组降低32.1%(P<0.01)、24.4%(P<0.05);提前给予大蒜油使小鼠4～14 h含量降低26%～54%不等,其中染毒后4、6、8、10 h与单纯染毒组比较,差异有统计学意义(P<0.05或P<0.01),但大蒜油并未改变正己烷染毒小鼠2,5-HD含量曲线的形状和达峰时间。结论大蒜油可抑制正己烷染毒小鼠2,5-HD的产生,降低血清2,5-HD水平,降低正己烷毒性。
Objective To investigate the effect of garlic oil on reducing the production of n-hexane metabolites in mice and provide experimental evidence for reducing n-hexane poisoning. Methods Healthy adult Kunming mice were randomly divided into n-hexane exposure group and garlic oil intervention group, n-hexane, garlic oil and n-hexane were intragastrically administered respectively. Blood samples were collected and the serum was separated by ethyl acetate extraction and determined by gas chromatography Serum 2,5-hexanedione (2,5-HD) content. Results After a single oral gavage with n-hexane, the content of 2,5-HD in serum was (0.14 ± 0.14) μg / mL and peaked at 10 h (24.63 ± 3.52) μg / mL, (0.02 ± 0.04) μg / mL before and after n-hexane administration. The serum 2,5-HD level of 6 h mice were decreased by 32.1% (P <0.01) and 24.4% <0.05). The garlic oil was given in advance to reduce the content of 4-14 h in mice by 26% -54%, and the difference was statistically significant at 4, 6, 8 and 10 h after exposure P <0.05 or P <0.01). However, garlic oil did not change the shape and peak time of 2,5-HD content curve in n-hexane-treated mice. Conclusions Garlic oil can inhibit 2,5-HD production in n-hexane-treated mice, decrease serum 2,5-HD level and decrease n-hexane toxicity.