单疱病毒性角膜炎是常见的致盲眼病 ,因潜伏感染 ,疾病反复发作 ,临床上单凭病变形态和临床经验诊断已不利于治疗。本文采用聚合酶链反应结合荧光探针的体外ＤＮＡ扩增和ＦＱ -ＰＣＲ检测技术对 4 2名临床诊断为病毒性角膜炎病人的 4 5份泪液进行病毒性角膜炎基因诊断 ,现总结 Herpes simplex virus keratitis is a common cause of blind eye, due to latent infection, recurrent disease, clinically based on the lesion morphology and clinical experience has been diagnosed with adverse treatment. In this paper, polymerase chain reaction with fluorescent probe in vitro DNA amplification and FQ-PCR detection of 42 clinically diagnosed patients with viral keratitis in 45 cases of viral keratitis gene diagnosis, are summarized