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目的快速准确检测广东地区广泛分布的主要HCV基因型,为HCV的临床诊断和治疗提供依据。方法收集53例HCV-RNA阳性血清标本,选取丙型肝炎病毒(HCV)基因组保守序列为扩增靶序列,设计通用引物和特异型别探针(1,2,3,6型),通过逆转录将RNA逆转录为cDNA后,利用通用引物对cDNA进行PCR扩增,将扩增产物利用反向点杂交(RDB)技术进行基因分型。结果HCV-RNA阳性血清53例,共检出标本52例,4种基因型所占的比例为1b型32例占60.38%,2a型4例占7.55%,3b型8例占15.09%,6a型8例占15.09%。结论所测患者的HCV基因型分布以1b为主,6a和3b次之,2a相对较低;反向点杂交技术能对其进行快速分型,符合率高达98.11%。
Objective To rapidly and accurately detect the major HCV genotypes widely distributed in Guangdong and provide evidence for the clinical diagnosis and treatment of HCV. Methods Fifty-three HCV-RNA positive serum samples were collected and the conservative sequence of hepatitis C virus (HCV) was selected as target sequence. Universal primers and specific probes (type 1, 2, 3 and 6) After transcribing RNA into cDNA, universal primers were used to amplify the cDNA. The amplified products were genotyped by reverse dot blot hybridization (RDB). Results Fifty-three HCV-RNA positive sera were detected in 52 cases. The proportion of 4 genotypes was 1b in 32 cases (60.38%), type 2a (7.55%), type 3b (15.09%) in 6 cases 8 cases accounted for 15.09%. Conclusions The genotypes of HCV genotypes were mainly 1b, 6a and 3b, and 2a were relatively low. Reverse dot blot hybridization could rapidly genotype HCV genotypes with the highest coincidence rate of 98.11%.