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目的研究木犀草苷对人食管鳞状癌Eca109细胞生长的抑制作用及其机制。方法 Eca109细胞经不同浓度木犀草苷处理后,MTT法检测Eca109细胞增殖;倒置显微镜观察Eca109细胞形态的变化;流式细胞术检测Eca109细胞周期变化及细胞凋亡情况;RT-PCR检测Eca109细胞cyclin D1、survivin和c-myc基因表达。结果 MTT实验表明,木犀草苷80、120、160、200、240μmol/L对Eca109细胞均有抑制作用,且与浓度相关。木犀草苷可引起Eca109细胞形态改变、体积缩小,并与周围细胞脱离,浓度为240μmol/L时使细胞呈出芽状,有的细胞伸出多个伪足样突起;浓度为160、240μmol/L时,可将细胞阻滞于G2/M期,诱导细胞凋亡;浓度为240μmol/L并处理Eca109细胞48 h后,使Eca109细胞cyclin D1、survivin和c-myc基因表达低于对照组(P<0.05)。结论木犀草苷对食管鳞状癌Eca109细胞的生长有显著抑制作用,其通过改变细胞周期、诱导细胞凋亡及抑制相关基因的表达发挥抗肿瘤作用。
Objective To study the inhibitory effect of luteolin on the growth of human esophageal squamous carcinoma Eca109 cells and its mechanism. Methods The Eca109 cells were treated with different concentrations of luteolin. The proliferation of Eca109 cells was detected by MTT assay. The morphology of Eca109 cells was observed by inverted microscope. The cell cycle and apoptosis of Eca109 cells were detected by flow cytometry. The cyclin of Eca109 cells was detected by RT-PCR. D1, survivin and c-myc gene expression. Results MTT assay showed that luteolin 80, 120, 160, 200, and 240 μmol/L all had inhibitory effects on Eca109 cells and were related to the concentration. Luteolin could induce morphological changes and decrease in size of Eca109 cells and detached from surrounding cells. The concentration of 240 μmol/L made the cells bud-like, and some cells protruded from many pseudopod-like processes; the concentration was 160,240 μmol/L. The cells can be arrested in the G2/M phase and induce apoptosis; the concentration of 240μmol/L and treatment of Eca109 cells for 48 h, the expression of cyclin D, survivin and c-myc genes in Eca109 cells was lower than that in the control group (P <0.05). Conclusion Luteolin has a significant inhibitory effect on the growth of esophageal squamous cell carcinoma Eca109 cells, which plays an anti-tumor role by changing the cell cycle, inducing apoptosis and inhibiting the expression of related genes.