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目的观察急性时相血清淀粉样蛋白A(A-SAA)和罗格列酮(RGZ)对内皮细胞白细胞介素(IL)6和IL-8基因表达的影响。方法分别用不同浓度(0.5μg/ml、5μg/ml)的A-SAA、RGZ(10μmol/L)处理ECV304内皮细胞8h、24h后抽提总RNA,用RT-PCR技术检测IL-6、IL-8 mRNA的表达。结果 A-SAA能显著促进ECV304细胞IL-6、IL-8 mRNA的表达,并呈剂量和时效依赖性(P<0.01)。而RGZ并不影响A-SAA对ECV304细胞IL-6和IL-8 mRNA表达的促进作用。结论 A-SAA可通过促进内皮细胞IL-6、IL-8的表达和分泌,参与动脉粥样硬化的形成;RGZ并非通过影响A-SAA对内皮细胞IL-6和IL-8表达的促进作用发挥其抗炎、抗动脉粥样硬化的作用。
Objective To observe the effects of acute phase serum amyloid A (A-SAA) and rosiglitazone (RGZ) on the expression of interleukin (IL) 6 and IL-8 in endothelial cells. Methods ECV304 endothelial cells were treated with different concentration (0.5μg / ml, 5μg / ml) of A-SAA and RGZ (10μmol / L) for 8h respectively. Total RNA was extracted 24 hours later and detected by RT-PCR. -8 mRNA expression. Results A-SAA could significantly promote the expression of IL-6 and IL-8 mRNA in ECV304 cells in a dose-and time-dependent manner (P <0.01). However, RGZ did not affect A-SAA to promote IL-6 and IL-8 mRNA expression in ECV304 cells. Conclusions A-SAA can participate in the development of atherosclerosis by promoting the expression and secretion of IL-6 and IL-8 in endothelial cells. RGZ does not affect the effect of A-SAA on the expression of IL-6 and IL-8 in endothelial cells Play its anti-inflammatory, anti-atherosclerotic effect.