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[目的]研究免疫性肝纤维化大鼠肝组织基质金属蛋白酶9(MMP-9 mRNA)和基质金属蛋白酶组织抑制因子1(TIMP-1 mRNA)基因表达的动态变化及下瘀血汤对其影响,探讨下瘀血汤抗肝纤维化的机制。[方法]猪血清腹腔注射复制大鼠肝纤维化模型,造模8周后灌胃给予下瘀血汤流浸膏干预治疗,用药4周后和造模过程中的1、2、4、8周动态处死大鼠,获取标本,检测指标。[结果]与正常组相比,模型组1、8、12周MMP-9 mRNA表达明显降低(P<0.05,<0.01),2周MMP-9 mRNA表达显著升高(P<0.01),4周表达无明显变化。与模型组12周相比,下瘀血汤组MMP-9 mRNA表达明显升高(P<0.05)。与正常组相比,1、2、4、8和12周模型组TIMP-1 mRNA表达均有显著升高(P<0.05,<0.01))。与12周模型组相比,下瘀血汤组TIMP-1 mRNA表达显著降低(P<0.01)。[结论]大鼠猪血清免疫性肝纤维化存在着MMP-9 mRNA和TIMP-1 mRNA的动态变化,下瘀血汤可通过促进MMP-9 mRNA表达和抑制TIMP-1 mRNA表达而发挥抗肝纤维化的作用。
[Objective] To investigate the dynamic changes of gene expression of matrix metalloproteinase 9 (MMP-9 mRNA) and tissue inhibitor of metalloproteinase 1 (TIMP-1 mRNA) in liver tissues of rats with autoimmune liver fibrosis and the effect of Xiayuxue decoction To investigate the mechanism of Xiayu Decoction against hepatic fibrosis. [Method] The rat model of hepatic fibrosis induced by intraperitoneal injection of porcine serum was established. After 8 weeks of modeling, the rats were treated with Xiaoyu Tang Decoction by intragastric administration. After 4 weeks of treatment, Week rats were sacrificed dynamically to obtain specimens, indicators. [Results] Compared with the normal group, the expression of MMP-9 mRNA in the model group was significantly lower at 1, 8, 12 weeks (P <0.05, <0.01) No significant changes in the expression of the week. Compared with the 12-week model group, MMP-9 mRNA expression was significantly increased in Xiaoyuxue decoction group (P <0.05). Compared with the normal group, TIMP-1 mRNA expression in the 1,2,4,8 and 12-week model groups were significantly increased (P <0.05, <0.01). Compared with the 12-week model group, the expression of TIMP-1 mRNA in Xiayuxuexue Decoction group was significantly decreased (P <0.01). [Conclusion] The dynamic changes of serum MMP-9 mRNA and TIMP-1 mRNA in rat serum autoimmune liver fibrosis can be observed. Xiayuxue decoction can exert anti-liver activity by promoting the expression of MMP-9 mRNA and inhibiting the expression of TIMP-1 mRNA The role of fibrosis.