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应用电子自旋 (ESR)波谱术研究了同步辐射辐照种子产生的自由基及其与辐射损伤之间的关系。结果表明 ,同步辐射辐照的种子产生了大量的自由基 ,其种类、性质不同于γ射线 ,短寿命自由基 (小于 48h)产额高于γ射线 ,长寿命自由基 (大于48h)的稳定性大于γ射线。同步辐射自由基产额与辐照剂量呈线性关系。用3H TdR掺入法研究了受辐照种胚萌发早期DNA的合成动态 ,结果表明 ,同步辐射能够引起种胚DNA的损伤修复合成 ,其特点不同于γ射线。原冬 6号和北京41 1品种的DNA修复合成出现在种胚吸水后第一个正常的DNA合成峰之前 ,京冬8号品种出现在第一个小峰之后和 2个高峰之前
The electron spin (ESR) spectroscopy was used to study the relationship between free radicals produced by irradiated seeds and their effects on radiation damage. The results showed that seeds irradiated by synchrotron radiation produced a large number of free radicals. The types and properties of radionuclides were different from that of γ-rays. The yield of short-lived free radicals (less than 48h) was higher than that of γ-rays and long-lived free radicals Sex is greater than gamma rays. The synchrotron radiation free radical yield and irradiation dose have a linear relationship. The 3H TdR incorporation method was used to study the synthesis of DNA at the early germination stage of radiated embryos. The results showed that synchrotron radiation could induce DNA damage repair and synthesis, which is different from γ ray. DNA repair synthesis of Haemon 6 and Beijing 41 1 appeared before the first normal DNA synthesis peak after embryo imbibition, and the Jingdong 8 variety appeared after the first small peak and before the two peaks