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目的:探讨DNA错配修复基因hMSH6及PTEN表达与40岁以下子宫内膜样腺癌(EEC)的关系。方法:采用免疫组化EnVision法检测hMSH6、PTEN在40例40岁以下EEC组织的表达,并选取40岁以下子宫内膜单纯性增生13例,子宫内膜复杂性增生12例,子宫内膜不典型增生11例为对照。结果:hMSH6、PTEN在子宫内膜单纯性增生、复杂性增生、不典型增生、EEC的表达均呈递减趋势(P=0.002,P=0.005);EEC hM-SH6、PTEN表达率虽低于子宫内膜不典型增生,但差异无统计学意义。hMSH6表达与手术病理分期和子宫肌层浸润有关(P=0.045,P=0.038);PTEN蛋白表达与组织分级有关(P=0.01)。hMSH6与PTEN蛋白在子宫内膜组织的表达呈正相关(rs=0.96,P<0.05)。hMSH6、PTEN均表达阳性的EEC组织分化好于均表达阴性者,病理分期早。结论:hMSH6、PTEN基因功能缺失与EEC的早期发生有关,40岁以下EEC hMSH6与PTEN蛋白表达缺失者有预后不良的倾向。
Objective: To investigate the relationship between DNA mismatch repair gene hMSH6 and PTEN expression and endometrial adenocarcinoma (EEC) under 40 years of age. Methods: Immunohistochemical EnVision method was used to detect the expression of hMSH6 and PTEN in 40 cases of EEC under the age of 40, 13 cases of simple endometrial hyperplasia under 40 years old, 12 cases of endometrial hyperplasia, 12 cases of endometrial hyperplasia Typical hyperplasia in 11 cases as a control. Results: The expression of hMSH6 and PTEN in endometrial hyperplasia, complex hyperplasia and atypical hyperplasia showed a decreasing trend (P = 0.002, P = 0.005). Although the expression rate of hM-SH6 and PTEN in EEC was lower than that in uterus Intimal atypical hyperplasia, but the difference was not statistically significant. The expression of hMSH6 was correlated with pathological stage and myometrial invasion (P = 0.045, P = 0.038). PTEN protein expression was correlated with histological grade (P = 0.01). There was a positive correlation between hMSH6 and PTEN expression in endometrial tissue (rs = 0.96, P <0.05). HMSH6, PTEN positive expression of EEC tissue differentiation was better than those who were negative, pathological staging as early as possible. CONCLUSION: The loss of function of hMSH6 and PTEN genes is related to the early development of EEC. The prognosis of patients with EEC under 40 years old with impaired expression of hMSH6 and PTEN tend to be poor.