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目的调查贵州地区各类深部感染来源的白念珠菌CAI区微卫星基因型分布特点。方法收集95株贵州地区深部感染来源的白念珠菌,采用CAI区的单链构型多态性(SSCP)图谱结合GeneScan分析进行CAI区微卫星分型;计算多态性信息含量(PIC)和Nei氏遗传距离,以类平均法(UPGMA)进行聚类分析。结果 95株贵州地区深部感染来源的白念珠菌被鉴定为40种CAI区微卫星基因型,其中有7种属至今尚未报道过的CAI区微卫星基因型;PIC为0.9046;根据白念珠菌CAI区微卫星基因型间的Nei氏遗传距离将菌株分为7大类群,其中慢性阻塞性肺疾病和获得性免疫缺陷综合征(AIDS)来源白念珠菌部分菌株在某一类群呈集中分布,其余部分菌株在各类群中呈散在分布,而肿瘤来源菌株则在聚类分析中呈相对集中分布的特点。结论贵州地区深部感染不同来源的白念珠菌菌株具有丰富的遗传多样性;不同来源的白念珠菌的CAI区微卫星基因型在微生态进化上具有相关性。
Objective To investigate the distribution of microsatellite genotypes of Candida albicans CAI in various types of deep-seated origin in Guizhou province. Methods A total of 95 strains of Candida albicans were collected from Guizhou province. The single nucleotide polymorphism (SSCP) maps of CAI region and GeneScan were used to analyze the microsatellite loci in CAI region. The polymorphism information content (PIC) and Nei’s genetic distance, cluster analysis using UPGMA. Results 95 strains of Candida albicans in Guizhou province were identified as 40 CAI microsatellite genotypes, of which 7 were microsatellite genotypes in CAI regions that have not been reported so far. The PIC was 0.9046. According to the results of C. albicans CAI The Nei’s genetic distance among the microsatellite genotypes in the district was divided into seven groups. Among them, some strains of Candida albicans isolated from chronic obstructive pulmonary disease and Acquired Immunodeficiency Syndrome (AIDS) were concentratedly distributed in one group, while the rest Some strains were scattered in various groups, while the tumor-derived strains were relatively concentrated in the cluster analysis. CONCLUSION: Candida albicans strains from different origins in Guizhou Province are rich in genetic diversity. Microsatellite genotypes of CAI in Candida albicans from different origins are related to the evolution of micro-organisms.