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目的:研究牛蒡子苷与牛血清白蛋白(BSA)的相互作用机制。方法:采用紫外-可见吸收光谱法和同步荧光光谱法。结果:在△λ=15 nm时,牛蒡子苷对8SA的荧光具有规律性增强作用,最大发射波长蓝移,表明牛蒡子苷的加入影响了BSA中酪氨酸残基的微环境。用荧光效应增强方程计算它们在298 K、310 K和318 K温度下的结合常数分别为K_1=7.899×10~4L/mol、K_2=5.962×10~4L/mol和K_3=3.903×10~4L/mol,对应温度下的热力学参数△H=-26.874 kJ/mol,△S分别为3.601、4.737、3.395 J/(mol·K),△G分别为-27.940、-28.340、-27.951 kJ/mol。结论:牛血清白蛋白与牛蒡子苷分子间有较强的结合作用,且结合力以静电相互作用为主。
Objective: To study the interaction between arctiin and bovine serum albumin (BSA). Methods: Ultraviolet-visible absorption spectroscopy and synchronous fluorescence spectrometry were used. Results: At △ λ = 15 nm, arctigen enhanced the fluorescence of 8SA regularly and the maximum emission wavelength shifted blue, indicating that arctiin affected the microenvironment of tyrosine residues in BSA. Their binding constants at 298 K, 310 K and 318 K were calculated as K 1 = 7.899 × 10 4 L / mol, K 2 = 5.962 × 10 4 L / mol and K 3 = 3.903 × 10 4 L, respectively, using the fluorescence enhancement equation / mol, the thermodynamic parameters △ H = -26.874 kJ / mol and ΔS were 3.601, 4.737 and 3.355 J / mol · K, respectively, ΔG were -27.940, -28.340 and -27.951 kJ / mol . Conclusion: Bovine serum albumin and arctiin molecules have strong binding, and the binding force is mainly electrostatic interaction.