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采用聚合酶链反应技术对四川地区2003~2004年收集的60例宫颈癌患者的癌组织DNA进行人乳头瘤病毒(HPV)E6基因扩增,获得HPV18和45型E6基因.序列分析发现,18型三例E6基因有同样的两处同义突变;45型两例E6基因发生突变,一例有两处碱基突变,另一例发生六处碱基突变,其中两处涉及氨基酸变化,均位于E6抗原决定簇区.HPV45型E6基因中134位c→t,157位c→t,259位g→t和341位t→c的碱基点突变未见报道.另外,该地区HPV18和45型突变株之间存在碱基互变,它们之间的最小差异比野生型HPV18和HPV45之间的差异小4.05%,该数值比在非洲发现的突变株的要小很多,该结果支持HPV18和HPV45可能起源于非洲的观点.
Polymerase chain reaction (PCR) was used to amplify human papillomavirus (HPV) E6 gene in 60 cases of cervical cancer tissue DNA collected in Sichuan Province from 2003 to 2004, and obtained HPV18 and 45 E6 genes.Sequence analysis found that 18 Type E6 gene three identical synonymous mutations; 45 two cases of E6 gene mutation, a case of two base mutations, another case of six base mutations, two of which involved in amino acid changes, are located in the E6 Antigenic determinant region.HPV45 E6 gene at 134 c → t, 157 c → t, 259 g → t and 341 t → c base point mutation has not been reported.In addition, the region HPV18 and 45 There was base-to-base interconversion between the mutants, with a minimum difference of 4.05% less than that between wild-type HPV18 and HPV45, a much smaller value than the mutant strains found in Africa, supporting HPV18 and HPV45 It may have originated in Africa.