A new chiral stationary phase of 3, 5-dimethylphenylcarbamates of cellulose,chemically bonded to 3-aminopropylsilica gel at the 6-positions of the glucose units, was prepared.The solvent versatility of the CSP was investigated for the enantioselective separation of four pairs of enantiomers using THF and chloroform as non-standard mobile phase eluent in HPLC. The influence of temperature on the resolution was investigated.